Answer Best answer as chosen by user Bethington OTA update will unroot your phone.
Yes, the OTA update will unroot your phone. However, industrious fellows are at work at this very moment to provide you with new, "stock" rooted ROMs that will consist of this latest software version (meaning whatever is applied in the OTA update) as well as the basics for rooting (superuser, busybox, various other apps the developer decides to include).
A quick trip to google ("new evo root ROM" or something similar) should provide you with a few links to stock root ROMs. As an alternative if you don't like using ROMs, you can go back to factory defaults (which will remove root, I think, as well as any and all data and apps you have), or somehow unroot (Google is your friend, again) it, apply the update, then reroot it.
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